Changes in Absorbance of Monolayer of Living Cells Induced by Laser Radiation at 633, 670, and 820 nm

Absorption changes in HeLa cells were evaluated from 530-890 nm. Cytochrome c oxidase becomes more oxidized (implying increased cellular oxidative metabolism) at all wavelengths used. This study supports the hypothesis that the mechanism of photobiomodulation is related to the chromophore cytochrome c.

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